Mutated Yeast Heat Shock Transcription Factor Activates Transcription Independently of Hyperphosphorylation

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The yeast and mammalian Ras pathways control transcription of heat shock genes independently of heat shock transcription factor.

Yeast strains in which the Ras-cyclic AMP (cAMP) pathway is constitutively active are sensitive to heat shock, whereas mutants in which the activity of this pathway is low are hyperresistant to heat shock. To determine the molecular basis for these differences, we examined the transcriptional induction of heat shock genes in various yeast strains. Activation of heat shock genes was attenuated i...

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Complex regulation of the yeast heat shock transcription factor.

The yeast heat shock transcription factor (HSF) is regulated by posttranslational modification. Heat and superoxide can induce the conformational change associated with the heat shock response. Interaction between HSF and the chaperone hsp70 is also thought to play a role in HSF regulation. Here, we show that the Ssb1/2p member of the hsp70 family can form a stable, ATP-sensitive complex with H...

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Antiproliferative prostaglandins activate heat shock transcription factor.

Treatment of human K562 erythroleukemia cells with the antiproliferative prostaglandin A1 results in the elevated transcription of two heat shock genes, HSP70 and HSP90. Parallel with increased heat shock gene transcription is the activation of heat shock transcription factor. Heat shock transcription factor levels are induced within 60 min after prostaglandin A1 addition to levels similar to t...

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Oxidative Injury Rapidly Activates the Heat Shock Transcription Factor but Fails to Increase Levels of Heat Shock Proteins1

When cells are exposed to heat shock, heavy metals, amino acid ana logues, and other stresses, the heat shock transcription factor (HSF) is activated. The HSF then binds to the promoter of the heat shock genes, stimulating transcription of the heat shock proteins. Here, we demonstrate that exposure of NIH-3T3 cells to oxidanls iI1..O, or menadione) also causes activation of the HSF. This activa...

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Factor but Fails to Increase Levels of Heat Shock Proteins Oxidative Injury Rapidly Activates the Heat Shock Transcription

When cells are exposed to heat shock, heavy metals, amino acid ana logues, and other stresses, the heat shock transcription factor (HSF) is activated. The HSF then binds to the promoter of the heat shock genes, stimulating transcription of the heat shock proteins. Here, we demonstrate that exposure of NIH-3T3 cells to oxidanls iI1..O, or menadione) also causes activation of the HSF. This activa...

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 2006

ISSN: 0021-9258

DOI: 10.1074/jbc.m510827200